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1、FX-5000T細胞應(yīng)力加載系統(tǒng)(flexcell FX5000 Tension system)
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| 1）該系統(tǒng)對二維、三維細胞和組織軸向和圓周應(yīng)力加載； |
| 2）基于柔性膜基底變形、受力均勻;
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| 3）可實時觀察細胞、組織在應(yīng)力作用下的反應(yīng);
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| 4）可有選擇性地封阻對細胞的應(yīng)力加載;
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| 5）同時兼?zhèn)浼毎麎毫虞d功能;
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| 6）多達4通道，可4個不同程序同時運行；
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| 7）同一程序中可以運行多種頻率，多種振幅和多種波形；
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| 8）更好地控制在超低或超高應(yīng)力下的波形；
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| 9）多種波形種類：靜態(tài)波形、正旋波形、心動波形、三角波形、矩形以及各種te制波形；
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| 10)電腦系統(tǒng)對牽張拉伸力加載周期、大小、頻率、持續(xù)時間精確智能調(diào)控
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| 應(yīng)用范圍：加載分析各種細胞在應(yīng)力刺激下的生物化學(xué)反應(yīng)，
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| 例如：骨骼細胞、肺細胞、心肌細胞、血細胞、皮膚細胞、肌腱細胞、韌帶細胞、軟骨細胞和骨細胞、
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| 腎膀胱細胞、平滑肌細胞/尿路上皮及尿路上皮細胞、眼上皮細胞、
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| 眼小梁組織細胞、腎小管上皮細胞、腸上皮細胞、胃上皮細胞等細胞牽張拉伸力加載 |
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2、FX-5000C細胞壓力加載系統(tǒng)(flexcell FX5000 Compression system)
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| 1）該系統(tǒng)對各種組織、三維細胞培養(yǎng)物提供周期性或靜態(tài)的壓力加載； |
| 2）基于柔性膜基底變形、受力均勻; |
| 3）可實時觀察細胞、組織在壓力作用下的反應(yīng); |
| 4）可有選擇性地封阻對細胞的應(yīng)力加載; |
| 5）同時兼?zhèn)浼毎麪繌埩虞d功能; |
| 6）多達4通道，可4個不同程序同時運行； |
| 7）同一程序中可以運行多種頻率(0.01- 5 Hz)，多種振幅和多種波形； |
| 8）更好地控制在超低或超高應(yīng)力下的波形； |
| 9）多種波形種類：靜態(tài)波形、正旋波形、心動波形、三角波形、矩形以及各種te制波形； |
| 10)電腦系統(tǒng)對壓力加載周期、大小、頻率、持續(xù)時間精確智能調(diào)控
應(yīng)用范圍： |
| 檢測各種組織和細胞在壓力作用下的生物化學(xué)反應(yīng)， |
| 例如：胃上皮細胞、腸上皮細胞、軟骨組織，椎間盤骨組織，肌腱 |
| 組織，韌帶組織，以及從肌肉、肺（肺細胞）、心臟、血管、皮膚、肌腱、韌帶、軟骨和骨中分離出來的細胞 |
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3、Flex Jr. Tension顯微應(yīng)力加載系統(tǒng)(Flex Jr. Tension) |
| 該系統(tǒng)對顯微鏡下的細胞提供雙軸向應(yīng)力或單軸向應(yīng)力加載。 |
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重點應(yīng)用范圍： |
| 心臟、骨骼肌和平滑肌，肺、血管內(nèi)皮細胞、皮膚、腱、韌帶、軟骨、骨細胞機械拉伸力加載分析 |
| 4、Tissuetrain三維細胞組織培養(yǎng)系統(tǒng)(flexcell Tissue®train System) |
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系統(tǒng)功能亮點： |
| .1)三維細胞應(yīng)力刺激：對生長在三維狀態(tài)下的細胞進行靜態(tài)的或者周期性的應(yīng)力刺激. |
| .2)三維細胞培養(yǎng)：使用三維組織培養(yǎng)模具和三維細胞培養(yǎng)板可以進行三維細胞培養(yǎng). |
| .3)三維細胞應(yīng)力加載： |
| 通過flexcell應(yīng)力加載系統(tǒng)和弧矩形加載平臺對生長在三維環(huán)境下的細胞進行單軸向或者雙軸向的靜態(tài)或者周期性的應(yīng)力加載實驗.
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| 4)動力模擬實驗:可建立te制的各種模擬實驗：心率模擬實驗，步行模擬實驗，跑動模擬實驗和其他動力模擬實驗. |
| 5)生物人工組織構(gòu)建：可構(gòu)建長度達35mm的生物人工組織 |
| 6)觀察細胞應(yīng)力下實時反映：使用標(biāo)準(zhǔn)正立式顯微鏡實時觀察細胞在三維狀態(tài)下的反應(yīng). |
| 7）多種基質(zhì)蛋白包被的尼龍網(wǎng)錨可以加強細胞與網(wǎng)錨的結(jié)合. |
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5、Str-4000細胞流體切應(yīng)力系統(tǒng)(flexcell Fluid Shear Stress Device) |
| 該系統(tǒng)為細胞提供各種形式的流體切應(yīng)力：穩(wěn)流式切應(yīng)力，脈沖式切應(yīng)力或者往返式切應(yīng)力，并能通過te顯微設(shè)備實時觀察細胞在機械力刺激下的反應(yīng). |
| 美國Glycotech經(jīng)濟、簡便平行板流動小室（Parallel-Plate Flow Chamber）
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Vacu-Cell™ Cell Perfusion and Incubation Chambers |
| 二、瑞士InSphero簡便、無需支架GravityPLUS三維培養(yǎng)平臺 |
| The industry-leading solution for reliable, scaffold-free 3D cell-based assays |
| ●Proven reliability and ease of use with many liquid-handling systems |
| ● SureDrop™ technology assures precise microtissue size – always! |
| ● Includes the GravitytrAP™ plate for long-term culture, assays and imaging |
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三、美國- Inc,MIC-101簡便、經(jīng)濟低氧、高氧培養(yǎng)設(shè)備 |
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成立于1971年美國比盧普斯羅森堡公司(－)的缺氧、常氧、高氧模塊化孵化室系統(tǒng) |
| 已用于世界各地四十年。 |
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該公司生產(chǎn)的缺氧\高氧培養(yǎng)系統(tǒng)可自定義的混合氣體和溫度組織培養(yǎng)條件.密閉室已被證明安、可靠， |
| 每個單元是一個自包含的孵化器，使您能夠創(chuàng)建所需的組織培養(yǎng)的各種環(huán)境 (氣體濃度和濕度)， |
| 已廣泛應(yīng)用于北美各大實驗室和研究機構(gòu)。該產(chǎn)品獲美國，在已發(fā)表的論文中引用率在同類產(chǎn)品中是屬于高的， |
| 并逐漸被球的實驗研人員所使用。 |
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四、英國solentim的Cell Metric和Cell Metric CLD設(shè)備
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英國精密儀器制造商Solentim公司已于5月9日推出了新細胞株發(fā)展(CLD)成像分析系統(tǒng)Cell Metric。 |
| 據(jù)悉，這款具有高分辨率的te細胞成像分析系統(tǒng)為手動檢查細胞株單克隆性提供了一種可替代方法， |
| 即對單個細胞克隆進行快速辨識和有效跟蹤。此外，自動對焦是Cell Metric性能發(fā)揮和確保圖像明確且一貫信息的保障。 |
| 另據(jù)了解，Solentim公司是一家致力于細胞生物學(xué)實驗室自動化儀器開發(fā)創(chuàng)新的英國公司。 |
| 該團隊在生物學(xué)識別和解決工作流程方面具有多年的經(jīng)驗。 |
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A fully automated bench-top system for measuring visual, non-invasive cell content of adherent mammalian cells. |
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Images are acquired at single cell resolution for whole microtitre plates using an integrated digital microscope. Cells are imagedin situ, |
| meaning they are not stressed by sample removal (pipetting or enzymic treatement), and since no fluorescent dyes or stains have been added these cells are physiologically relevant for time course studies. |
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Images are processed and presented on the computer with analysis of direct cell number, |
| confluence, colony count or morphology differentiation. Data can be exported and a digital record is created. |
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Key application workflows where Cell Metric™ delivers significant benefits include: |
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Single cell cloning, clonality and cell line development
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| Compound screening, cell proliferation and metabolism |
| Colony counting and clonogenic assays |
| Tissue engineering and stem cells
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A dedicated new high-resolution bench-top imaging system with integrated plate loader, specifically developed to speed up cell line development (CLD).
The unique cell imaging capabilities of the Cell Metric CLD™ enable fast, unequivocal identification and tracking of single-cell derived clones.
Find out more about the key features:
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| Generate cell lines faster |
| Verify mono-clonality |
| Save resources and money |
| Integrated plate loader |
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五、美國動態(tài)血腦屏障平臺（blood-brain barrier (BBB)）
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Introduction to the Blood-Brain Barrier
TThe blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. |
| The BBB is formed by brain capillaries that look like simple tubes of endothelial cells. |
| Experimental and clinical evidence suggests that the BBB maintains the chemical environment for neuronal function and protects the brain from harmful substances. |
| The blood-brain barrier is therefore crucial for complex issues such as drug delivery, pathogenesis of chronic neurological diseases, and issues related to bio-defense. |
| We have developed a practical in vitro model that accurately reflects BBB characteristics. |
Benefits of ’s in vitro BBB Model |
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In contrast to other available models of the blood-brain barrier, 's Dynamic In vitro Blood-Brain Barrier (DIV-BBB) respects the anatomical aspects of the in situ endothelial |
| cell-astrocyte interactions and replicates the physiological levels of shear stress to which in situ endothelial cells are exposed. |
| The DIV-BBB allows formation of physiological transendothelial resistance, and formation of gap junctions that can be easily visualized |
| at the electron microscope or determined experimentally with the use of tracers.
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六、美國InQbio細胞生長、培養(yǎng)、成像、監(jiān)控多功能綜合系統(tǒng)
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InQ Cellular Research System |
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The InQ Cell Research System is the first solution that integrates a dynamic software-controlled lab incubator with advanced live cell imaging and real-time data collection. |
| It creates a truein vivoenvironment for studying sensitive human and animal cells for disease research. |
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The system's exclusive capability to tightly control environmental parameters with correlated sample feedback creates a new paradigm in stem cell, neuroscience, |
| and other cell-based research. Scientists will be empowered with novel and more relevant approaches to discovery as they pursue the causes of devastating diseases.
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