Fibronectin is an attachment factor that facilitates the attachment and cytoplasmic spreading of all types of anchorage-dependent cells. Fibronectin is responsible for cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis.

Plasma fibronectin (pFN) is secreted by hepatocytes as a major protein component of blood. pFN opsonizes bacteria and helps form clots. Plasma FN is widely used in cell culture to help cells attach to the substrate. It is particularly useful for culturing cells that are incapable of synthesizing their own biomatrix, including stem cells, and for culturing cells in serum-free medium. Akron’s pFN is a clear, sterile, buffered solution with a pH of about 7.0 to 8.0.

Advantages:

• Rapid attachment
• High plating and cloning efficiencies
• Good proliferation, high saturation density
• Lower requirements for serum and added growth factors
• Better response to physiologically occurring hormones, expression of differentiated functions
• Cells have longer life span, flattening and morphological changes, and improved plating consistency

Research Application:

• ELISA
• Immunoblotting
• Cell culture substratum
• Stem cell research