| neuroindx品牌Unipick單細胞抓取系統(tǒng),UnipicK單細胞捕獲抓取系統(tǒng) | ||||||||
| 型號:Unipick，Unipick plus | ||||||||
| 價格:請致電：010-67529703 | ||||||||
| 品牌:neuroindx | ||||||||
UnipicK單細胞捕獲抓取系統(tǒng)
——多用途和高性價比的細胞采集和組織顯微切割系統(tǒng) 產(chǎn)品簡介： 目前仍wu實用快速的技術(shù)可以用來從細胞培養(yǎng)直接采集感興趣的單細胞用作下游單細胞分析和再培養(yǎng)。同樣，異質(zhì)性組織采集te異性的組織區(qū)域或單細胞仍然具有挑戰(zhàn)性?，F(xiàn)有的技術(shù)，包括流式細胞篩選和激光輔助的技術(shù)，都很昂貴和復(fù)雜，并且在使用上有各樣的局限性。UnipicK是由美國NIH資助的新的單細胞和組織采集系統(tǒng)，經(jīng)過5年研發(fā)，由美國NEUROINDX研發(fā)生產(chǎn)。該系統(tǒng)性能te，使用專利保護的脈沖負(fù)壓為動力，玻璃毛細管為工具，可以和大部分倒置顯微鏡配合使用，可以快速和j確地直接從常規(guī)細胞培養(yǎng)板采集單細胞或細胞克隆，并且對細胞活性影響輕微。采集的細胞可以再培養(yǎng)，和用作下游各種單細胞分析。 由于UnipicK采集后的單細胞仍需要手動通過微小注射器來釋放出細胞，所以2017年1月NEUROINDX推出了具有自動采集和自動釋放單細胞的新一代單細胞和組織挑取系統(tǒng)：UnipicK Plus。經(jīng)過萊比信的反復(fù)驗證，確定其可以自動將采集后的細胞放到旁置的PCR管中，PCR管架具有48孔，可以滿足絕大多數(shù)客戶的需求。 UnipicK及UnipicK Plus系統(tǒng)均可以從細胞培養(yǎng)體系和復(fù)雜異質(zhì)性組織，比如懸浮細胞體系、三維培養(yǎng)的組織體系、腦組織、病理切片等，根據(jù)組織病理學(xué)，熒光標(biāo)記和解剖學(xué)需要來采集單細胞和te異性的解剖學(xué)區(qū)域。UnipicK及UnipicK Plus采集的樣品可廣泛用于下游眾多領(lǐng)域的現(xiàn)代分子生物學(xué)技術(shù)，te別是針對蛋白質(zhì)和核酸，包括但不限于定量 RT-PCR，基因表達，表觀遺傳學(xué)和蛋白質(zhì)組學(xué)研究等。系統(tǒng)本身較已有的系統(tǒng)相比價格低，一機多用，經(jīng)濟實惠，性價比高，容易使用，簡單快捷，耗材便宜，采集成本低。儀器不需要專門的維護，結(jié)實耐用。
一次性毛細管針頭直徑在15-100μm之間，滿足不同大小的細胞的選取 可供采集毛細管內(nèi)徑大?。?span>15，20，30，40，50，60，80，100 μm 主要應(yīng)用領(lǐng)域 原代組織細胞培養(yǎng)、回收 干細胞的有效分選研究 分離人的單克隆抗體 癌癥研究與治療 基因組學(xué)和蛋白質(zhì)組學(xué)分析 檢測及分離懸浮培養(yǎng)液中的稀有細胞，如腫瘤循環(huán)細胞CTC 生物芯片細胞分選 單細胞PCR和基因表達分析 腫瘤干細胞的分離 動物腫瘤模型分析 主要特點 操作簡便，容易上手 單細胞分辨率 組織顯微切割 可采集貼壁，懸浮和三維細胞培養(yǎng) 采集的細胞保持高活性 高品質(zhì) RNA和蛋白質(zhì)用于下游各種組學(xué)分析 靈活性和多用途 經(jīng)濟實惠，高性價比 采集成本低 儀器主要勢 系統(tǒng)操作：配備高精度機械移動電機，保證微量毛細管位于視場的正中心 毛細管內(nèi)徑范圍：15~100um，可給予客戶的應(yīng)用提供的內(nèi)徑選擇方案 組織樣品的厚度： 10~300um，可直接從載玻片上進行細胞分選 針對細胞種類：血液、骨髓、制備的各種組織細胞懸浮液,任何單一類型細胞或、稀有細胞(如循環(huán)腫瘤細胞、內(nèi)皮細胞、干細胞以及血液、骨髓中相關(guān)細胞等) 樣品采集過程：不涉及化學(xué)，熱，激光和輻射處理，對細胞影響很小，保證細胞的活性和完整性，所分離的組織或細胞可以提取高質(zhì)量的 DNA， RNA 和蛋白質(zhì)，供下游研究使用 工作效率：一般的分選過程只需幾分鐘就可完成 客戶反饋 UnipicK是多用途的產(chǎn)品—可以從細胞培養(yǎng)和組織切片采集樣品 UnipicK具有靈活性—和幾乎鎖有倒置顯微鏡兼容v UnipicK具有的回退功能，多孔培養(yǎng)板仍然可以暢通wu阻 UnipicK具有再校對功能—實驗過程更高效，更快速 相關(guān)文獻 Xu MJ, Cooke M, Steinmetz D, Karakousis G, Saxena D, Bartlett E, et al. (2015) A Novel Approach for the Detection and Genetic Analysis of Live Melanoma Circulating Tumor Cells. PLoS ONE 10(3): 0123376. doi:10.1371/journal.pone.0123376 Vi N, Ma Z, Fields T, Avliyakulov NK, Haykinson MJ, Bragin A, and Karsten SL (2012) Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues. PloS One. 7(7):41564. 使用客戶 清華大學(xué)，武漢大學(xué)，浙江大學(xué)，復(fù)旦大學(xué)，同濟大學(xué)，廈門大學(xué)，中山大學(xué)，中國科學(xué)院有機化學(xué)研究所，神經(jīng)科學(xué)研究所，國家蛋白質(zhì)科學(xué)中心，南方科技大學(xué)，深圳人民醫(yī)院轉(zhuǎn)化醫(yī)學(xué)中心，愛爾眼科學(xué)研究所，香港城市大學(xué)，香港中文大學(xué)，澳門大學(xué)
UNIPICK
UnipicK is a vacuum-assisted single cell collection and tissue microdissection instrument based on NeuroInDx’s original capillary-based cell and tissue acquisition system (CTAS; USPT 8,797,644; Kudo et al, 2012) that can acquire individual cells from cell cultures and tissues. It permits rapid acquisition of single cells from any untreated cell cultures, providing reliable and affordable means of most sophisticated experimentation. Moreover, the instrument is suitable for individual cell collection and region specific acquisition of subanatomical areas from complex heterogeneous tissues such as the brain. Operation of the system is intuitive and requires minimal training. Cell cultures require no treatment or specific peripherals, thus enabling unmatched flexibility in experimental design and sample collection.
Perhaps one of the most distinct features is the ability of UnipicK™ to collect live cells for further reculturing and a plethora of downstream functional studies. Both individual fluorescently labeled and morphologically distinct cells can be acquired from any cell cultures or tissue sections. The procedure has been demonstrated from nearly all types of adherent, suspended and 3D cultures, including difficult samples such as primary cells, or individual cells from fresh frozen and sucrose treated complex tissues, such as brain (please, seehttp://www.youtube.com/user/NDXInc). With live cells viability tests have demonstrated up to 99% after collection, and up to 30 cells per minute may be collected from adherent cultures. RNA isolated from collected cells and tissue regions exhibits high integrity and low degradation rates with RIN >9.0. Subsequent labeling protocols (e.g. T7 based) consistently produce high incorporation yields making it suitable for downstream PCR, microarray and sequencing analyses. The instruments’ low cost, ease of use, single cell resolution, and minimal impact on cell viability, make it unique and vital research tools. Importantly, UnipicK™ is a free standing instrument that can be mounted over a wide range of inverted microscope models, providing additional flexibility in the laboratory.
Notable Features of UnipicK™ include: – Simple operation with minimal training – Single cell collection from untreated cultures and tissue sections – Efficient tissue microdissection – High viability of collected cells – High quality RNA and protein for downstream –omics studies – Flexibility and versatility – fit any inverted microscope – Fraction of the cost of any laser assisted microdissection instrument
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