產(chǎn)品名稱Biochips芯片
品牌cellix
產(chǎn)品貨號Biochips芯片
產(chǎn)品價格現(xiàn)貨詢價
聯(lián)系人李先生
聯(lián)系電話18618101725
產(chǎn)品說明



Biochips

Cell Adhesion & Cell Culture under Flow, Transmigration, Droplet Generation and Microfluidics Studies



一、在剪切流下細胞-配體/細胞-蛋白粘附芯片
一次性生物芯片
細胞粘附、配體/蛋白質(zhì)包被通道上的滾動檢測
非常適合血小板聚集和血栓形成分析。 


Cell-Ligand / Cell-Protein adhesion 
Under shear flow
Disposable biochip
Cell adhesion, rolling assays on ligand/protein coated channels
Ideal for platelet aggregation & thrombosis assays.

二、在剪切流下細胞培養(yǎng) ＆細胞間粘附芯片
一次性生物芯片
微通道流室中內(nèi)皮細胞單層的細胞培養(yǎng)
細胞-細胞粘附剪切流分析 


Cell culture &cell-cell adhesion
Under shear flow
Disposable biochip
Cell culture of endothelial cell monolayers in microchannel flow chambers
Cell-cell adhesion shear flow assays

三、在剪切流下細胞-配體和細胞-細胞粘附芯片

一次性生物芯片
玻璃蓋玻片隨附提供的靈活性。
玻璃蓋玻片可以用螯合的 Cu2+ 離子連接到高密度 PEG 涂層上處理 
Cell-Ligand &cell-cell adhesion
Under shear flow
Disposable biochip
Flexibility with glass coverslip supplied unattached.
Glass coverslips may be treated with chelated Cu2+ ion tethered to the high density PEG-coating
四、VenaDelta Y1模擬分支微血管網(wǎng)絡分支Y型結點在輸入端口芯片

趨化性測定、雙流、多層流和擴散 
Branching Y-junction at input port
Mimic branching microvascular networks
Chemotaxis assays, dual flow, multilaminar flow & diffusion

五、VenaDelta Y2 模擬分支微血管網(wǎng)絡，分支 Y 型接頭在輸入和輸出端口


趨化性測定、雙流、多層流和擴散 
VenaDelta Y2 Branching Y-junction
at input & exit ports
Mimic branching microvascular networks
Chemotaxis assays, dual flow, multilaminar flow & diffusion
六、VenaT4趨化性，輪回，侵襲試驗芯片


在剪切流下
剪切流下的細胞粘附和遷移
穿過微孔膜進入可能含有化學引誘劑的下層微孔 
Chemotaxis, Transmigration, Invasion Assays
Under shear flow
Cell adhesion and transmigration under shear flow
Transmigration through microporous membrane into underlying microwell which may contain chemoattractant
七、在剪切流下液滴生成芯片

3 個液滴發(fā)生器和 1 個分流器
微滴和單分散油/乳液的高通量生成 
Droplet Generation
Under shear flow
3 Droplet Generators & 1 splitter
High-throughput generation of microdroplets and monodispersed oils/emulsions

HOW DOES IT WORK?

DropChips are fabricated from plastic (acrylic) and have a footprint of 40mm x 50mm (W x L).

DropChips are available with channels pre-treated as hydrophobic or hydrophilic:

  • Hydrophobic to achieve water-in-oil droplets.

  • Hydrophilic to achieve oil-in-water droplets.

 

DropChips are available in packs of 2, 5 or 10.

Hydrophobic channels:

Water-in-oil droplet generation

Hydrophilic channels:

Oil-in-water droplet generation

As illustrated in the schematic below, each DropChip contains:

  • 1 x Flow Splitter and

  • 3 x Droplet Generator junctions:

    • 2 x Flow-Focusing junctions, single-injection dispersed phase

    • 1 x Flow Focusing junction with dual-injection dispersed phase

This gives the user flexibility for experiments in flow focusing with single and dual-injection of dispersed phase.

Examples are illustrated in the images below.

Droplet Frequency

The creation of uniform, mono-dispersed  stable droplets is achieved with microfluidic syringe pumps such as Cellix's ExiGo pump or microfluidic pressure pumps such as Cellix's UniGo or 4U pumps.  Cellix's team conducted 3D scattering measurements to confirm frequency of droplets which can be achieved at defined flow rates as illustrated in the images and frequency graph below.  If you have a specific frequency you want to achieve, contact us for more help.

Droplet Frequency in DropChip  with channel dimensions 70um x 70um

Droplet Size

There are a variety of different droplet sizes possible and one of the biggest influencing factors of droplet size is the geometry and size of the channel dimensions.  Cellix's team conducted 3D scattering measurements to confirm size and volume of droplets which can be achieved with the two different types of droplet generator contained in the DropChip.  Schematics for each of the droplet generators available on Cellix's Dropchip are illustrated below and the following table provides a summary of the droplet sizes (diameter and volume) which may be achieved depending on the DropChip dimensions.

Table_Droplet Sizes_DG1 and DG3.jpg
Droplet Generator 1 and 3_Flow Focusing.

Droplet Generator junction 1 & 3 (DG1 and DG3):

Flow Focusing, single injection of dispersed phase

Droplet Generator 2_Co-flow Focusing.jpg

Droplet Generator junction 2 (DG2):

Flow Focusing, dual injection of dispersed phase

Table_Droplet Sizes_DG2.jpg
Table_DropChip_Flow Splitter Dimensions.
 
 
 

APPLICATIONS

Droplet Generation for a wide variety of applications:

  • Cell / Protein encapsulation and sorting on-chip, see example below.

  • DNA sequencing for target selection / enrichment.

  • Sample / reagent mixing and control of reactions on-chip

  • DNA amplification

  • Biomolecule synthesis

  • Diagnostic chips

  • Drug discovery

For more details on experimental set-ups, visit our Droplet Generation page.

APPLICATION VIDEOS

Cell (or particle) double encapsulation:

The image below illustrates droplets containing cells, enclosed within a shell of oil.  The following is an example of the experimental set-up to achieve double encapsulation.

Experimental Set-up

  • 3 x ExiGo pumps

  • 3 x Flow Sensors

  • 1 x DropChip (50um x 50um) coated with DropCoat (reagent ensuring hydrophobicity)

  • 1 x DropChip (80um x 80um) uncoated, hydrophilic walls

Table_Cell double encapsulation example.
 

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