Description

Endocan is a proteoglycan composed of a 20 kDa mature polypeptide and a 30 kDa chondroitin /?dermatan sulfate chain.

It circulates in the blood to the average concentration of 1 ng / mL.

Key identified biological activities :

• increases endothelial migration induced by VEGF,
• comitogen with HGF / SF and FGF-2,
• protumoral,
• anti-inflammatory by inhibiting leukocyte activation cascade LFA-1-dependent.

Characteristics

• Recombinant human Endocan expressed by a human cell line type HEK293
• Produced in bioreactor in fetal calf serum free medium
• Purified by ion exchange and immunoaffinity chromatographies
• PM: 50 kDa in western blot
• Format: 10 μg
• Purity: > 90% polyacrylamide gel revealed with silver nitrate
• Storage at -80°C in phosphate buffer without preservatives

Documentation

• Datasheet
• Examples of protocols
• Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, Vazeux R, Richet C, Degand P, Jude B, Janin A, Fernig DG, Tonnel AB, Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001;276(51):48341-9
• Adam E, Sarrazin S, Landolfi C, Motte V, Lortat-Jacob H, Lassalle P, Delehedde M. Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor.Biochem Biophys Res Commun. 2008;369(2):297-302

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