產(chǎn)品詳情
Human endocan recombinant 20 kDa (50 μg)
Purified human Endocan, non-glycosylated.
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This reference ( LIP-1002-50U) is only intended for?research (RUO / research use only) . And not for diagnostic or therapeutic uses.
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Mutation of the single glycosylation Site (S137A) prevents the formation of the glycan chain endocan.
This mutation generates a non-glycosylated 20 kDa protein corresponding to the body of the molecule.
Key identified biological activities :
Anti-tumor,?
Pro-inflammatory.
Recombinant human S137A Endocan expressed by a human cell line type HEK293
Produced in bioreactor in fetal calf serum free medium
Purified by ion exchange and ?immunoaffinity chromatographies
PM: 20kDa Western blot
Format: 50 μg
Purity: > 90% polyacrylamide gel revealed by silver nitrate
Storage at -80 ° C in phosphate buffer without preservatives
Applications: bioassays
Datasheet Examples of protocols Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, Vazeux R, Richet C, Degand P, Jude B, Janin A, Fernig DG, Tonnel AB, Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001;276(51):48341-9 Adam E, Sarrazin S, Landolfi C, Motte V, Lortat-Jacob H, Lassalle P, Delehedde M. Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor.Biochem Biophys Res Commun. 2008;369(2):297-302 Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35
Visit our webpages? Clinicals Areas ?and? Endocan ?for more informations.
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Endomark? H1 :?LIDM-1201
Anticorps anti-endocan humain (N-Ter) MEP21 100 μg :?LIA-0902
Anticorps anti-endocan humain (C-Ter) MEP14 100 μg :?LIA-1001
Do It Yourself ELISA Kit H1 (Endocan humain) :?LIK-1101
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This reference can be also ordered in superior quantity (bulk). Do not hesitate to contact us for any questions and/or quotation request at?This email address is being protected from spambots. You need JavaScript enabled to view it. .
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