Description

The DIY (Do it Yourself) Elisa Kit R1 or DIYEK R1 (ref LIK-1103) is an immunoassay enables 88 samples in duplicate to be analyzed in two 96-well plates.

Simply have 100 μL of serum, plasma or culture supernatant of rat cells to perform this assay.

The DIYEK R1 (LIK-1103) contains the essential compounds to allow for the quantification of Endocan in biological fluids (serum, plasma, etc.) and in the culture supernatants :

• One vial of anti-Endocan monoclonal antibody, capture antibody,
• One vial of anti-Endocan monoclonal antibody, detection antibody,
• Two vials of lyophilized recombinant mouse/rat Endocan for quantification

The protocol (inserted into the kit and downloadable from our website) is easily achievable and provides highly reproducible results.

You can also buy the mouse Endocan independently for your plate patterns (companion product: LIP-1104).

Characteristics

• Sandwich ELISA using two monoclonal antibodies purified by affinity
• Detects the mouse Endocan
• Format: 2 x 96 wells
• Duration: 4 hours
• Linearity: 0.3 to 10 ng / ml
• Detection limit: 0.3 ng / ml
• Limit of quantification: 0.6 ng / ml
• Inter Series reproducibility (% CV): 9.19%
• Within run repeatability (% CV): 5.79%

Documentation

• Instructions
• Examples of Protocols

• Depontieu F, de Freitas Caires N, Gourcerol D, Giordano J, Grigoriu B, Delehedde M, Lassalle P. Development of monoclonal antibodies and ELISA specific for the mouse vascular endocan. J Immunol Methods. 2012;378(1-2):88-94
• Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35.

Check our pages Clinical areas and Endocan for more information.

Complementary products

• Anti-endocan antibody mouse (N-Ter) 100 μg GGR222: LIA-0905
• Anti- endocan antibody human/mouse/rat (C-Ter) 100 μg MEP14: LIA-1001
• Anti-endocan antibody mouse/rat (Internal) 100 μg GGR237: LIA-1101
• Standard mouse Endocan: LIP-1104