| 產(chǎn)品詳情 |
Humain endocan recombinant 50 kDa (10 μg)
Purified human Endocan containing the glycan chain type chondroitin / dermatan sulfate.
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This reference ( LIP-1001-10U) is only intended for research (RUO / research use only). And not in diagnostic or therapeutic uses.
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Endocan is a proteoglycan composed of a 20 kDa mature polypeptide and a 30 kDa chondroitin /?dermatan sulfate chain.
It circulates in the blood to the average concentration of 1 ng / mL.
Key identified biological activities :
- increases endothelial migration induced by VEGF,
- comitogen with HGF / SF and FGF-2,
- protumoral,
- anti-inflammatory by inhibiting leukocyte activation cascade LFA-1-dependent.
- Recombinant human Endocan expressed by a human cell line type HEK293
- Produced in bioreactor in fetal calf serum free medium
- Purified by ion exchange and immunoaffinity chromatographies
- PM: 50 kDa in western blot
- Format: 10 μg
- Purity: > 90% polyacrylamide gel revealed with silver nitrate
- Storage at -80°C in phosphate buffer without preservatives
- Datasheet
- Examples of protocols
- Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, Vazeux R, Richet C, Degand P, Jude B, Janin A, Fernig DG, Tonnel AB, Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001;276(51):48341-9
- Adam E, Sarrazin S, Landolfi C, Motte V, Lortat-Jacob H, Lassalle P, Delehedde M. Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor.Biochem Biophys Res Commun. 2008;369(2):297-302
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