Description

The DIY (Do it Yourself) Elisa Kit M1 or DIYEK M1 (ref LIK-1102) is an immunoassay enables 88 samples in duplicate to be analyzed in two 96-well plates.

Simply have 100 μL of serum, plasma or culture supernatant of murine cells to perform this assay.

The DIYEK M1 (LIK-1102) contains the essential compounds to allow for the quantification of Endocan in biological fluids (serum, plasma, etc.) and in the culture supernatants:

• One vial with anti-human Endocan monoclonal antibody, capture antibody,
• One vial with anti-human monoclonal antibody Endocan, detection antibody,
• Two vials of lyophilized recombinant mouse Endocan for quantification.

The protocol (inserted into the kit and downloadable from our website) is easily achievable and provides highly reproducible results.

You can also buy the mouse Endocan independently for your plate patterns (companion product: LIP-1104).

Characteristics

• Sandwich ELISA using two monoclonal antibodies purified by affinity
• Detects the human and monkey Endocan
• Format: quantity sufficient for 2 x 96 wells
• Duration: 4 hours
• Linearity: 0.3 to 10 ng / mL
• Detection limit: 0.3 ng / ml
• Limit of quantification: 0.6 ng / ml
• Inter Series reproducibility (% CV): 9.19%
• Within run repeatability (% CV): 5.79%

Documentation

• Instructions
• Examples of Protocols

• Depontieu F, de Freitas Caires N, Gourcerol D, Giordano J, Grigoriu B, Delehedde M, Lassalle P. Development of monoclonal antibodies and ELISA specific for the mouse vascular endocan. J Immunol Methods. 2012;378(1-2):88-94
• Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35.

Check our pages Clinical areas and Endocan for more information.

Complementary products

• Anti-endocan antibody mouse (N-Ter) 100 μg GGR222: LIA-0905
• Anti- endocan antibody human/mouse/rat (C-Ter) 100 μg MEP14: LIA-1001
• Anti-endocan antibody mouse/rat (Internal) 100 μg GGR237: LIA-1101
• Standard mouse Endocan: LIP-1104