TBARS Background

2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.? TBARS assay values are usually reported in malonaldehyde (MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides.? The TBARS assay is a well recognized, established method for quantifying these lipid peroxides.

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However, because typical TBARS assays utilize high temperatures in the presence of strong acids, TBA has been found to react with many interfering compounds, which has been a long standing criticism of this method.? Non-specific interferences that contribute to overall absorbance measurements plague the traditional TBARS assay by yielding artificially high results when measuring MDA concentrations.

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Oxford Biomedical Research has developed an Enhanced TBARS Assay that shortens the reaction time and uses a proprietary polyacid, greatly reducing the non-specific interfering signals.? This results in a more accurate and reliable MDA measurment.

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Advantages

• TBARS assay uses a polyacid to enhance MDA measurement
• Has a reduced background signal
• Can be run in just 15 minutes
• Can be used with any biological, nutraceutical or food sample
• Less expensive
• Available in cuvette and microplate formats
• No boiling tubes with marbles

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