GST Alpha ELISA

Glutathione S-Transferase (GST) has multiple isoforms. This assay is specific for Glutathione S-Transferase Alpha (GSTA) and is not known to cross react with the mu, pi, or theta variants. GSTA is a common biomarker for hepatocellular damage.¹ It also conjugates GSH to 4-hydroxynonenal, a product of lipid peroxidation² and is an important player in cellular antioxidant defense mechanisms.³

This is a standard sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-GSTA and blocked, ready for the addition of samples and standards. The assay should take approximately 2.5 hours to run, plus any required sample preparation time.?

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When measuring urine samples, it is recommended to normalize samples to creatinine to account for variations in fluid intake and excretion. ?Creatinine is produced at a constant rate and can be used to normalize the concentrations of other analytes. ?Creatinine Assay Kit (CR01).



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References

1. Vaubourdolle, M, et al., Clinical Chemistry 41: 1716-1719, 1995
2. Awasthi, Y. C, et al., Free Radic. Biol. Med. 37: 607–619, 2004
3. Yang, Y., et al. Toxicol. Appl. Pharmacol. 182: 105–115, 2002