The human cytosolic Glutathione S-Transferases (GSTs) are expressed as 18 distinct gene products yet they share a common structural morphology. This immunoassay is specific for GST Pi (GSTP) and exhibits less than 1% cross reactivity towards the other 17 human cytosolic GST enzymes. GSTP is considered to be a urinary biomarker for renal cell damage and is localized to the distal convoluted tubules, thin Loop of Henle, and the collecting ducts of the kidney.

This is a standard sandwich enzyme linked immunosorbent assay (ELISA). The plate is pre-coated with anti-GSTP and blocked, ready for the addition of samples and standards. The assay should take about three hours to run, plus any required sample preparation time.

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When measuring urine samples, it is recommended to normalize samples to creatinine to account for variations in fluid intake and excretion. ?Creatinine is produced at a constant rate and can be used to normalize the concentrations of other analytes. ?Creatinine Assay Kit (CR01).

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Typical Standard Curve