CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis.Cdc25, when phosphorylated on serine 216 by CHK1 becomes bound by an adaptor protein in the cytoplasm.Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1.Consequently, a cell is prevented from entering mitosis.CHK1 can also phosphorylate p53 at Ser20 in vitro.
| Target |
CHK1 |
| Reactivity |
Human, Mouse |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of CHK1 expressed in E. coli. |
| Purification |
Purified antibody |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
56 kDa |
| GeneID |
1111
|
| Concentration |
1.0 mg/ml |
| Buffer |
PBS containing 0.03% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |