PFN1 Antibody is a Rabbit Polyclonal antibody against PFN1. The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8).

Target	PFN1
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB
Recommended dilutions	WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Recombinant protein of human PFN1.
Purification	Affinity purified.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 15 kDa Observed MW: 16 kDa
Swiss Prot	P07737
GeneID	5216
Gene Symbol	PFN1
Concentration	> 1 mg/ml
Buffer	PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.