p53 (pS15) Antibody is a Rabbit Polyclonal antibody against p53 (pS15). This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277).

Target	p53 (pS15)
Reactivity	Human
Host	Rabbit
Modification	Phosphorylation
Target Modification	Ser15
Clonality	Polyclonal
Tested Applications	WB, IF/ICC
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/20 - 1/50. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding S15 of human p53.
Purification	Affinity purified.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 23-43 kDa Observed MW: 55 kDa
Swiss Prot	P04637
GeneID	7157
Gene Symbol	TP53
Concentration	> 1 mg/ml
Buffer	PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.