The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily.

The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily. The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA is then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5-10ml of starting material. The use of a novel binding membrane makes it possible to isolate up to 40μg of pDNA.