Unique buffer and stabilizers have been optimized exclusively for SYBR? Green I qPCR to deliver maximum PCR efficiency, sensitivity, and a robust fluorescent signal

Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX? reference dye. Platforms not requiring passive reference dye include Roche LightCycler? 400, Bio-Rad? iQ?, MyiQ?, iQ?5, Opticon?, and Chromo4?, Corbett Rotor-Gene?, Eppendorf? Mastercycler?, and Cepheid? SmartCycler?. Kits with Low ROX? reference dye are for use with platforms including AB 7500 and Stratagene? Mx?. Kits with ROX? reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne?.

All SuperMixes are available with or without uracil-N-glycosylase (UNG) for prevention of carry-over contamination from previous dU containing PCRs.

Highly specific amplification is crucial to successful qPCR with SYBR? Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart? Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.