產(chǎn)品名稱 illustra plasmidPrep Midi Flow Kit, GE Healthcare
產(chǎn)品貨號 28-9042-68
產(chǎn)品價格 現(xiàn)貨詢價，電話：010-67529703
產(chǎn)品規(guī)格
產(chǎn)品品牌 vwr
產(chǎn)品概述
產(chǎn)品詳情
illustra plasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 ml for high copy number plasmid DNA and up to 500 ml for low copy number plasmid DNA

  • Provides plasmid DNA with excellent purity and low endotoxin levels
  • Sample type E. coli cultures containing high, low or very low-copy number plasmids and cosmid vectors up to 50 kbp
  • Modified alkaline lysis procedure
  • Anion exchange chromatography
  • > 70% (strain-dependent) of supercoiled pDNA
  • \< 10 endotoxin units (EU) per μg of plasmid DNA
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
  • Purification format is single-use, gravity flow illustra Fast-Flow plasmid 250 columns
  • Prep time 215 minutes

High column capacity allows for a proportional yield for input sample volume of 25 to 50 ml (for high copy number plasmid) and up to 500 ml for low copy number plasmid. Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification

Kit consistently produces high yields of pure plasmid DNA with low levels of endotoxin contamination.

The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA. The kit contains fast-flow plasmid 250 columns, resuspension, lysis, neutralization, wash, elution, and TE buffers, and a full protocol booklet with a detachable, quick reference protocol card.

Ordering information: Kit contains lysis buffer type 11 (Red, 2 x 360 mL), lysis buffer type 12 (Clear, 2 x 360 mL), lysis buffer type 13 (Blue, 2 x 360 mL), wash buffer type 2 (Yellow, 5 x 480 mL), elution buffer type 3 (Silver, 480 mL), TE buffer type 1 (Green, 120 mL), illustra Fast-Flow plasmid 250 columns (2 x 50), column stand, waste reservoir and adapters (12).
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