產(chǎn)品名稱 Cell Stimulation Cocktail, Tonbo Biosciences
產(chǎn)品貨號(hào) TNB-4975-UL100
產(chǎn)品價(jià)格 現(xiàn)貨詢價(jià)，電話：010-67529703
產(chǎn)品規(guī)格
產(chǎn)品品牌 vwr
產(chǎn)品概述
產(chǎn)品詳情
Cell stimulation cocktail is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. It can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.

  • Should be stored at –20 °C
  • To be protected from light and tightly sealed to prevent evaporation

It has been quality-tested by treatment of mouse splenocytes for 6 to 18 hours followed by intracellular staining and flow cytometric analysis of IL-2 or Ifn-gamma. It should be used at 1X final concentration for cell stimulation. It is recommended to add the cocktail directly to the cell suspension at 2 uL/mL to obtain a 1X final concentration.

Application: Flow cytometry and functional assays.

Delivery information: Supplied as 500X solution in ethanol.
產(chǎn)品資料