Sample Type: Serum, Plasma, Biological Fluids Intended Use: This BioAssay? kit is a competitive ELISA for in vitro quantitative measurement of GT in rat serum, plasma and other biological fluids Sensitivity: 37.5pg/mL Range: 62.5-4000pg/mL Specificity: Rat Test Principle: This BioAssay? ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with rat GT (Gastrin). During the reaction, rat GT (Gastrin) in the sample or standard competes with a fixed amount of rat GT (Gastrin) on the solid phase supporter for sites on the Biotinylated Detection Ab specific to rat GT (Gastrin). Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wave