normal Human Dermal Fibroblasts (HDF) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our fibroblasts are quality tested in both LIFib Serum-Free Medium and LIFib S2 Medium to ensure optimal growth at rates equal to or greater than classical serum-supplemented medium. Cell Features? HDFn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded in culture vessels once before cryopreservation. HDFa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded in culture vessels once before cryopreservation. Human Lung Fibroblasts are cryopreserved as secondary cells cells are isolated from human lungs and expanded in culture vessels twice before cryopreservation. Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium. Fibroblasts are extensively tested to meet quality standards and exhibit optimal performance. Fibroblasts are suitable for use as feeder layers as xeno-free or non-xeno free cultures for human embryonic stem cells and other cell culture applications requiring feeder layers.