This ATAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATAb.ATAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Teichoic Acid antigen and an ATAb-HRP conjugate. The assay sample and buffer are incubated together with ATAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of th