R-Spondin 1 (RSPO1) BioAssay? ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of RSPO1 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 15.6-1000pg/ml Sensitivity: 6.3pg/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027789A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use *027789B: Standard, 2x1 vial 027789C: Standard Diluent, 1x10ml or 1x20ml *027789D: Detection Reagent A (green), 1x60ul or 1x120ul *027789E: Detection Reagent B (red), 1x60ul or 1x120ul 027789F: Assay Diluent A, 1x6ml or 1x12ml 027789G: Assay Diluent B, 1x6ml or 1x12ml 027789H: TMB Substrate, 1x4.5ml or 1x9ml *027789J: Positive Control, 146.4pg/ml, 1x1 vial 027789K: Stop Solution, 1x3ml or 1x6ml 027789L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (027789K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using th