The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of MTX in Chicken serum, plasma and other biological fluids This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Chicken MTX. During the reaction, Chicken MTX in the sample or standard competes with a fixed amount of Chicken MTX on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Chicken MTX. Excess conjugate and unbo