Purity: > 95% by SDS - PAGE Format: Liquid. In 50 mM HEPES (pH7.5) 150 mM NaCl, 1mM DTT, 10% glycerol . Human ubquitin-conjugating enzyme 7(ubcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes(E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human uBE2L1-uBE2L4 gene could potentially encode different isoforms of the ubcH7. uBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of ubcH7. ubcH7(154amino acid) was over-expressed in E.coli and purified by using conventional chromatography techniques.