Purity: > 85% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol . Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, Calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Recombinant human Calreticulin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.