Purity: > 95% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol, 0.1M NaCl.. PCOLCE, also known as procollagen C-endopeptidase enhancer 1, is a secreted extracellular matrix glycoprotein. This protein is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin. Also, fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. Also, this protein binds and drives the enzymatic cleavage of type 1 procollagen and heightens C-proteinase activity. Recombinant human PCOLCE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.