Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl. Fibroblast growth factor 1, also known as FGF1, belongs to the fibroblast growth factor family. It is a potent mitogenic agent. FGF1 has been detected in large amounts in the brain. Other cells known to express FGF1 include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant mouse FGF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.