Purity: > 90% by SDS - PAGE Format: Liquid. In Phosphate Buffer Saline (pH7.4) containing 10% glycerol, 1mM DTT . Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.