Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT . CITED2 is an important transcriptional cofactor involved in multiple organ development. It is a cAMP-responsive element-binding protein (CBP)/p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor (HIF)-1alpha through its competitive binding with HIF-1alpha to CBP/p300. CITED2 is necessary for mouse fetal liver hematopoiesis and is required for the proper formation of the hyaloid vasculature and for lens morphogenesis. Recombinant CITED2 protein was expressed in E.coli and purified by using conventional chromatography techniques.