Purity: > 90% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 100mM NaCl . CKMT2, as known as SMTCK, belongs to the ATP:guanido phosphotransferase family. SMTCK is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.