Human T Cells-Negatively Selected (HTC) are purified from healthy Donors and ready to use in studies of T cell biology. HTC can respond to mitogens or anti-CD3 antibodies with robust proliferation and secretion of interferonγ, tumor necrosis factor α and interleukin-6. HTC are cryopreserved immediately after isolation and purification to ensure preservation of all circulating T cell subpopulations and antigen specificities. Our HTC are quality tested via flow cytometry to ensure depletion on undesired cell types and are typically ≥80% CD3 positive. HTC are positive for CD3 and contain both CD4 and CD8 subsets. HTC are negative for other lineage markers such as CD14, CD19 and CD56