Purity: > 85% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 10% glycerol, 1mM DTT . PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the u2 small nuclear ribonucleoproteins complex (u2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the u2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. Recombinant human PHF5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Size
100ug, 20ug, 4ug
Concentration
n/a
Applications
SDS-PAGE
Other Names
PHD finger-like domain-containing protein 5A, PHD finger protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b