Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular). See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d . Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely - be low. Furthermore, Gli-2 expression is likely - be developmentally regulated and induced, making it difficult - detect in whole tissue homogenates. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27760825).
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Description
The GLI-2 Antibody from Novus Biologicals is a rabbit polyclonal antibody to GLI-2. This antibody reacts with human, mouse, primate. The GLI-2 Antibody has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular). Specificity: NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human GLI-2 (isoform a).
Other Names
GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2