Flow Cytometry. Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
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Description
The IFN-gamma R1/CD119 Antibody (BB1E-2) [PE/Cy7] from Novus Biologicals is a mouse monoclonal antibody to IFN-gamma R1/CD119. This antibody reacts with human, bovine. The IFN-gamma R1/CD119 Antibody (BB1E-2) [PE/Cy7] has been validated for the following applications: Flow Cytometry. Specificity: In Western Blotting only one protein band is stained corresponding to a MW of 43,000, which is equal to the MW for the glycosylated extracellular part of the human extra-cellular interferon-gamma receptor (fused to c-myc), and a MW of 27,000 corresponding to the non-glycosylated part (fused to c-myc)
Immunogen
Fusion protein of the extracellular part of the human interferon-gamma receptor (AA: Ala17-Gly245) to 11 AA of the c-myc proto-oncogene (for affinity purification).