Propidium Iodide (PI) binds to double-stranded DNA, but it can only cross the plasma membrane of nonviable cells. When viewed using a fluorescence microscope with a rhodamine filter, cells that have lost membrane integrity will show red PI staining throughout the nuclei. For analysis by flow cytometry, the PI staining can be monitored in FL2 channel.
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Other Names
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, PI