This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pentosidine (PTD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pentosidine (PTD) and unlabeled Pentosidine (PTD) (Standards or samples) with the pre-coated antibody specific to Pentosidine (PTD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each micr