Western Blot (WB), Immunofluorescence Microscopy (IF), ICC
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Description
Anti-GFAP. Glial Fibrillary Acidic Protein (GFAP) was found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kD protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Immunogen
This antiserum was made with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with purified GFAP from bovine spinal cord.