Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA)
Description
Purity > 90% as determined by SDS-PAGE. MW: 38.1kDa. Full Length: 1-203aa. N-terminal 6xHis-SUMO-tagged. Escherichia coli (strain K12). The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.