The human HPRG / HRG ELISA Pair Set is for the quantitative determination of human HPRG / HRG.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for HPRG / HRGcoated on a 96-well plate. Standards and samples are added to the wells, andany HPRG / HRG present binds to the immobilized antibody. The wells are washedand a horseradish peroxidase conjugated rabbit anti-HPRG / HRG polyclonal antibody is then added, producing an antibody-antigen-antibody sandwich. Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of HPRG / HRG present in the sample. To endthe enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.