The number of bands in this product is 6. Marker4 is ideal for determining the size of double-stranded DNA from 500 to 10,000 base pairs. The ladder consists of 6 linear double-stranded fragments. The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 μl loading, all fragments except 2,500bp are 50ng. The 2,500bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.