Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Biotech's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen Biotech. Repeated freezing and thawing of cells is not recommended.Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.