spleen cells were fused with FOX-NY myeloma cells; the antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions; thymidine and aminopterin are added to the culture medium to stabilize the presence of the immunoglobulin heavy chain locusTumorigenecity:Isoenzyme:IgG3; kappa light chainHistopathology:hybridoma; mouse(myeloma)Subculture:Split ratio:Media change:Reverse transcritase:Productionimmunoglobulin; monoclonal antibody; against phosphotyrosine