Purity: > 90% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol . EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.