Purity: > 95% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT. MAD2L1, also known as MAD2L1-binding protein, is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Recombinant human MAD2L1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.