Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Mes buffer (pH 6.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT. E3 ubiquitin ligase RNF4, also known as RNF4, contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Recombinant human RNF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.