Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT. HAAO is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. This protein catalyzes the synthesis of quinolinic acid (QuIN) from 3-hydroxyanthranilic acid. QuIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QuIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QuIN. Recombinant human HAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.