Purity: 90> % by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT . AMTN belongs to the amelotin family. It could be a cell adhesion protein involved in the maturation of tooth enamel. AMTN is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between ameloblasts and enamel mineral and it co-localizes with another recently described enamel protein, odontogenic ameloblast-associated protein (ODAM). Recombinant human AMTN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.