Purity: > 85 % by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) 1M urea, 5% Glycerol.. Human ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. This protein is glycosylated and has a fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. ANGPT1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. Recombinant human ANGPT1 protein was expressed in E.coli and purified by using conventional chromatography techniques.