Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT . Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. uSP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. Recombinant human uSP46 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.